Confocal Microscope LSM 5
LSM 5 a laser scanning confocal microscope of the fifth generation with a fully motorized scanning mode is located in the laboratory of Professor Michelle Arbeitman in RRI 203.
The confocal microscope can be used to obtain optical sections of fluorescently-labeled, fixed tissue samples. It can detect signals from green to far red. It has two photomultiplier tubes, to simultaneously detect different signals. The microscope is an upright microscope with 4 objectives ranging from 10X-63X.
It also records time series. The image data can be acquired in different orientations and can be reconstructed at different viewing angles without the need to remount specimen. LMS 5 also performs quantitative measurements of, for example, calcium concentrations and pH-values, areas and surface properties.
All moving components of LSM 5, such as emission filter wheels, main and secondary dichroic beam splitters, the pinhole and the mechanical attenuators for each laser line, are computer-controlled and take up the required position
making the set-up error free and without any need for cumbersome manual operation. The position can be reset automatically at the press of a button.
LSM 5 provides image sizes of up to 2,048x2,048 pixels, scan fields of unrivaled size along with maximum linearity, 12-bit resolution per channel, high scan speeds, high-sensitivity detectors (selected photomultipliers) and short light paths from the specimen to the detector.7
Precisely controlable laser intensities to protect specimens and reduce the bleaching of dyes
Multifluorescence images without cross talk between the channels
Specification
Microscopes Upright: Axioplan 2 MOT, Axioskop 2 MOT, Axioskop 2 FS MOT,
Inverted: Axiovert 100 M Side/BasePort
Accessories like ICS objectives and filter cubes can be used
z Drives DC servo or step motor, smallest steps starting at 25 nm
Fine focusing stage HRZ 200, total range 200 μm, smallest step 10 nm
XY stage Motorized XY scanning stage, smallest step 250 nm
Scan Module Two independent galvo scanning mirrors
Scan resolution 1x4 to 2048x2048 pixels, user definable
Scan speed 2x10 levels, line frequencies from 4 up to 1300 Hz; 0.4 s per frame 512x512 pixels
Scan zoom 0.7x to 8x, variable with steps of 0.1
Scan rotation Any angle, variable with steps of 1°
Scan field 18 mm diagonal in the primary image plane (with zoom factor 1x)
Pinhole One pinhole with variable diameter size, adjustable
Detection 1 or 2 confocal R/FL channels with built in highly sensitive photomultiplier tubes
1 external transmitted light channel (DIC capable) optional
Dynamic range 12 bit per channel
Laser module Ar laser 488, 514 nm, 25 mW - HeNe laser 543 nm, 1 mW
Laser lines (end of lifetime specifications)
Attenuation Individual and variable intensity control of all lines
Electronics’ module Control circuitry for microscope, laser and scan module
LSM 5 Control with built in high performance Digital Signal Processor (DSP)
For more information go to www.zeiss.com
Core Facility will help you with obtaining free downloads of manuals, tutorials and software.
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